BVS Paraguay

Aplicación de dos tipos de amastigotes de Trypanosoma cruzi de difernte virulencia en una Elisa para la enfermedad de chagas

Maldonado, Marisel; Ichinose, Yoshio; Samudio, Margarita; Rojas de Arias, Antonieta; Sakamoto, Makoto; Moreno Azorero, Ricardo; Kanabara, Hiroji.

Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell- free liquid mediun were comparatively evaluated as solid-phase of Elisa for Chagas' disease. Amastigotes were obtained from the high- and low-virulent clones of the Talahuen strain and fron the G-1 strain. They were sonicated a...

Seguimiento por PCR del tratamiento de un paciente chagástico agudo

Russomando, Graciela; Figueredo, Antonio; Samudio, Margarita; Sakamoto, Makoto; Morita, kouichi.

The polymerase chain reaction (PCR) was usedto evaluate the course of treatment of an acutely infected patient with Trypanosoma cruzi. The reactions were performed using a primer pair (TCZ1 and TCZ2) which prime the amplification of a highly repetive 195-bp T. cruzi genomic DNA. Treatment with benzonidaz...

Caracterización de cepas de Trypanosoma cruzi de Paraguay usando análisis con enzimas de restricción del ADN del Kinetoplasto

Mimori, Tatsuyuki; Maldonado, Marisel; Samudio, Margarita; Rojas de Arias, Antonieta; Moreno Azorero, Ricardo; Sakamoto, Makoto.

Strains of Trypanosoma cruzi isolate in Paraguay from patients and vectors were examiqned by restriction endonuclease analysis of K-DNA using Eco RI, Hae III, Hinf I, Msp I. Four different types of schizodeme profiles were identified in 11 isolates. The firs group has much simpler profiles than ther grou...